ecsA

168

ABC transporter (ATP-binding protein), mutation affects proteolysis by RasP (no processing of RsgI resulting in absence if SigI activity, RsiW, and FtsL)

Locus: BSU_10040

Molecular weight: 27.57 kDa

Isoelectric point: 5.64

Protein length: 247 aa

Gene length: 744 bp

Function: regulation of the secretion apparatus and of intra-membrane proteolysis

Product: ABC transporter (ATP-binding protein)

Essential: no

Synonyms: ecsA, prsT, ecs-26, yhaD

Genomic Context

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List of homologs in different organisms, belongs to COG1131 (Galperin et al., 2021)

Gene

Coordinates: 1,077,440  1,078,183

Phenotypes of a mutant

• no processing of RsiW and FtsL PubMed
• inactivation of ''ecsA'' reduces sporulation efficiency to 0.1% that of wild type cells PubMed
• a cwlO ecsA mutant is not viable, due to lack of expression of lytE PubMed
• ponA ecsA double mutants have a growth defect that can be suppressed by reduced fatty acid synthesis (mutations in accA, accB, accC, accD or downregulation of the FapR regulon due to specific mutations in fapR) PubMed

The protein

Protein family

• ABC transporter superfamily (according to UniProt)

Domains

• ABC transporter domain (aa 4-234) (according to UniProt)

Structure

• 4RVC (PDB) (from Geobacillus kaustophilus, 71% identity) PubMed
• P55339 (AlphaFold)

Paralogous protein(s)

• YthP

Localization

• membrane associated (via EcsB) PubMed

Expression and Regulation

Operons

• Genes: ecsA-ecsB-ecsC

  Description: PubMed

Additional information

• the mRNA is very stable (> 15 min) PubMed

Biological materials

Mutant

• BKE10040 (ecsA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGTTTCTCCCCTTATAG,  downstream forward: _UP4_GAGCTTACAAAGGAAGACGC
• BKK10040 (ecsA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAGTTTCTCCCCTTATAG,  downstream forward: _UP4_GAGCTTACAAAGGAAGACGC

References

Reviews

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Original Publications

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