dhbC

168

isochorismate synthase

Locus: BSU_31990

Molecular weight: 43.29 kDa

Isoelectric point: 5.17

Protein length: 398 aa

Gene length: 1197 bp

Function: biosynthesis of the siderophore bacillibactin

Product: isochorismate synthase

Essential: no

E.C.: 5.4.4.2

Synonyms: dhbC

Genomic Context

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List of homologs in different organisms, belongs to COG1169 (Galperin et al., 2021)

Gene

Coordinates: 3,290,289  3,291,485

Phenotypes of a mutant

• defective in biofilm formation, this can be suppressed by the addition of 2,3-dihydroxybenzoate to the medium PubMed

The protein

Catalyzed reaction/ biological activity

• Chorismate --> isochorismate (according to UniProt)

Protein family

• isochorismate synthase family (with MenF, according to UniProt)

Structure

• 3OS6 (PDB), (from B. anthracis, 56% identity, 70% similarity)
• P45744 (AlphaFold)

Modification

• phosphorylation on Ser-271 PubMed

Expression and Regulation

Operons

• Genes: dhbA-dhbC-dhbE-dhbB-dhbF-ybdZ

  Description: PubMed

  Regulation

  • induced by iron starvation (second wave to allow iron scavenging from the environment) (Fur) PubMed
  • expression is increased in the presence of pulchimerrin PubMed

  Expression

  • expression is heterogeneous PubMed

  Regulatory mechanism

  • Fur: repression, PubMed, in fur regulon
  • AbrB: repression, PubMed, in abrB regulon
  • Kre: repression, in kre regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigI: sigma factor, PubMed, in sigI regulon

  Additional information

  • the ''dhbA-dhbC-dhbE-dhbB-dhbF operon is strongly unregulated in a kre'' mutant PubMed

Biological materials

Mutant

• BKE31990 (dhbC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTCATGAACCTCCTTT,  downstream forward: _UP4_TGAACAGAATGAATTGCTGG
• BKK31990 (dhbC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTCATGAACCTCCTTT,  downstream forward: _UP4_TGAACAGAATGAATTGCTGG

References

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