cysC

168

adenylyl-sulfate kinase

Locus: BSU_15600

Molecular weight: 22.40 kDa

Isoelectric point: 5.08

Protein length: 197 aa

Gene length: 594 bp

Function: sulfate reduction and activation

Product: adenylyl-sulfate kinase

Essential: no

E.C.: 2.7.1.25

Synonyms: cysC, ylnC

Genomic Context

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List of homologs in different organisms, belongs to COG0529 (Galperin et al., 2021)

Gene

Coordinates: 1,633,369  1,633,962

The protein

Catalyzed reaction/ biological activity

• adenosine 5'-phosphosulfate + ATP --> 3'-phosphoadenylyl sulfate + ADP + H⁺ (according to UniProt)

Protein family

• APS kinase family (with YisZ, according to UniProt)

Structure

• 3CR8 (PDB) (from Thiobacillus denitrificans, 46% identity, 62% similarity) PubMed
• O34577 (AlphaFold)

Expression and Regulation

Operons

• Genes: cysH-cysP-sat-cysC-ylnD-sirB-ylnF

  Description: PubMed

  Regulation

  • induced by methionine starvation (S-box) PubMed
  • the S-box RNA is degraded by RNase Y PubMed

  Regulatory mechanism

  • CymR: transcription repression, [pubmed|], in cymR regulon
  • S-box: RNA switch, , the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed, in S-box

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-B367 (ylnC::erm), available at the NBRP B. subtilis, Japan
• BKE15600 (cysC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GCGATTTGTCACTGCCAGTC,  downstream forward: _UP4_TGAATATGATCTGCTGCGTT
• BKK15600 (cysC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GCGATTTGTCACTGCCAGTC,  downstream forward: _UP4_TGAATATGATCTGCTGCGTT

Labs working on this gene/protein

• [[Isabelle Martin-Verstraete]], Institute Pasteur, Paris, France

References

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