asnS

168

asparagyl-tRNA synthetase

Locus: BSU_22360

Molecular weight: 48.92 kDa

Isoelectric point: 5.12

Protein length: 430 aa

Gene length: 1293 bp

Function: translation

Product: asparagyl-tRNA synthetase

Essential: no

E.C.: 6.1.1.22

Synonyms: asnS

Genomic Context

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List of homologs in different organisms, belongs to COG0017 (Galperin et al., 2021)

Gene

Coordinates: 2,346,224 → 2,347,516

Phenotypes of a mutant

• essential PubMed, non-essential according to PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + L-asparagine + tRNA^Asn --> AMP + diphosphate + H⁺ + L-asparaginyl-tRNA^Asn (according to UniProt)

Protein family

• Class-II aminoacyl-tRNA synthetase family (according to UniProt)

Structure

• 1X56 (PDB) (from Pyrococcus horikoshii, 42% identity) PubMed
• P39772 (AlphaFold)

Expression and Regulation

Operons

• Genes: asnS-dnaD-nth-ypoC

  Description: PubMed

Biological materials

Mutant

• GP2934 (10 bp deletion at bp 1013) (no resistance) available in Jörg Stülke's lab
• BKE22360 (ΔasnS::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACATATTTCCCTCCAAAA,  downstream forward: _UP4_TAATACATTCAAATGAAACA
• BKK22360 (ΔasnS::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAACATATTTCCCTCCAAAA,  downstream forward: _UP4_TAATACATTCAAATGAAACA

References

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