AroH

• Description: chorismate mutase (isozymes 1 and 2)
  
  

Gene name	aroH
Synonyms
Essential	no
Product	chorismate mutase (isozymes 1 and 2)
Function	biosynthesis of aromatic amino acids
Gene expression levels in SubtiExpress: aroH
Metabolic function and regulation of this protein in SubtiPathways: aroH
MW, pI	14 kDa, 5.524
Gene length, protein length	381 bp, 127 aa
Immediate neighbours	trpE, aroB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

The gene

Basic information

• Locus tag: BSU22690

Database entries

• BsubCyc: BSU22690

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Chorismate = prephenate (according to Swiss-Prot)

• Protein family: BCKDHA family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: AroH-AroH

• Localization:

Database entries

• BsubCyc: BSU22690

• Structure: 2CHT (complex with a transition state analog), 1DBF

• UniProt: P19080

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:
  • aroF-aroB-aroH (according to DBTBS)
  • aroF-aroB-aroH-trpE-trpD-trpC-trpF-trpB-trpA-hisC-tyrA-aroE (according to DBTBS)

• Expression browser: aroH PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1441 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1006 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 804 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Y M Chook, H Ke, W N Lipscomb
Crystal structures of the monofunctional chorismate mutase from Bacillus subtilis and its complex with a transition state analog.
Proc Natl Acad Sci U S A: 1993, 90(18);8600-3
[PubMed:8378335] [WorldCat.org] [DOI] (P p)