argS

168

arginyl-tRNA synthetase, universally conserved protein

Locus: BSU_37330

Molecular weight: 62.55 kDa

Isoelectric point: 5.11

Protein length: 556 aa

Gene length: 1671 bp

Function: translation

Product: arginyl-tRNA synthetase

Essential: yes

E.C.: 6.1.1.19

Synonyms: argS

Genomic Context

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List of homologs in different organisms, belongs to COG0018 (Galperin et al., 2021)

Gene

Coordinates: 3,833,650  3,835,320

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + L-arginine + tRNA^Arg --> AMP + diphosphate + L-arginyl-tRNA^Arg (according to UniProt)

Protein family

• Class-I aminoacyl-tRNA synthetase family (according to UniProt)

Structure

• 3FNR (PDB) (from Campylobacter jejuni, 39% identity, 56% similarity)
• P46906 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Additional information

• universally conserved protein

Expression and Regulation

Operons

• Genes: argS

  Description: PubMed

• Genes: ywiB-argS

  Description: PubMed

Additional information

• the mRNA is cleaved by RNase III PubMed

Biological materials

Expression vectors

• pGP2897: expression of Strep-argS by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab

References

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