alaR

168

transcriptional regulator (Lrp family) of the alaR-alaT operon

Locus: BSU_31410

Molecular weight: 18.62 kDa

Isoelectric point: 4.89

Protein length: 166 aa

Gene length: 501 bp

Function: unknown

Product: transcription regulator (Lrp family)

Essential: no

Synonyms: alaR, yugG

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1522 (Galperin et al., 2021)

Gene

Coordinates: 3,226,933  3,227,433

The protein

Protein family

• (Lrp family)

Domains

• HTH asnC-type domain (aa 3-65) (according to UniProt)

Structure

• O05236 (AlphaFold)

Modification

• phosphorylated on Arg-79 PubMed

Expression and Regulation

Operons

• Genes: alaR-alaT

  Description: PubMed

  Additional information

  • A ncRNA is predicted between yugI and alaT PubMed

Biological materials

Mutant

• MGNA-A622 (yugG::erm), available at the NBRP B. subtilis, Japan
• BKE31410 (alaR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGATTCACACCTTTGC,  downstream forward: _UP4_AAAAGAATCGTGGTGTCACC
• BKK31410 (alaR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGATTCACACCTTTGC,  downstream forward: _UP4_AAAAGAATCGTGGTGTCACC

References

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