adk

168

adenylate kinase

Locus: BSU_01370

Molecular weight: 23.97 kDa

Isoelectric point: 4.45

Protein length: 217 aa

Gene length: 654 bp

Function: ADP formation

Product: adenylate kinase

Essential: yes

E.C.: 2.7.4.3

Synonyms: adk

Genomic Context

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List of homologs in different organisms, belongs to COG0563 (Galperin et al., 2021)

Gene

Coordinates: 145,877 → 146,530

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + AMP --> 2 ADP (according to UniProt)

Protein family

• adenylate kinase family (single member, according to UniProt)

Structure

• 1P3J (PDB) PubMed
• P16304 (AlphaFold)

Localization

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• Genes: rplO-secY-adk-map

  Description: NULL

  Regulation

  • RelA dependent downregulation (Class I) during stringent response PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response

  Sigma factors

  • SigA: sigma factor, PubMed (two promoters, about 140bp and 200bp upstream of the start codon), in sigA regulon

  Additional information

  • strongly expressed

• Genes: rpsJ-rplC-rplD-rplW-rplB-rpsS-rplV-rpsC-rplP-rpmC-rpsQ-rplN-rplX-rplE-rpsN-rpsH-rplF-rplR-rpsE-rpmD-rplO-secY-adk-map-oapB-infA-rpmJ-rpsM-rpsK-S56-rpoA-rplQ

  Description: PubMed

  Regulation

  • strongly repressed in response to glucose starvation in M9 medium PubMed
  • likely autorepression of operon expression upon binding of excess of one ribosomal protein to the untranslated region of the mRNA PubMed
  • expression is reduced in motile cells as compared to non-motile cells PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response

  Sigma factors

  • SigA: sigma factor, PubMed (two promoters, about 140bp and 200bp upstream of the start codon), in sigA regulon

  Additional information

  • term-seq has identified a potential novel regulatory RNA element (protein-dependent leader) including an intrinsic transcription terminator upstream of rpsJ PubMed

References

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