cmoI

168

glutaredoxin required for the conversion of S-methyl-cysteine to cysteine

Locus: BSU_29320

Molecular weight: 10.49 kDa

Isoelectric point: 7.11

Protein length: 93 aa

Gene length: 282 bp

Function: utilization of S-methyl-cysteine

Product: glutaredoxin

Essential: no

E.C.: null

Synonyms: cmoI, ytnI

Genomic Context

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List of homologs in different organisms, belongs to COG0695 (Galperin et al., 2021)

Gene

Coordinates: 3,003,049 → 3,003,330

Phenotypes of a mutant

• no growth with S-methyl cysteine PubMed

The protein

Catalyzed reaction/ biological activity

• oxidation of S-methyl-cysteine PubMed
• reduction of N-acetyl-l-cysteine sulfenic acid to N-acetyl-l-cysteine PubMed

Protein family

• glutaredoxin family (single member, according to UniProt)

Domains

• Glutaredoxin domain (aa 1-93) (according to UniProt)

Structure

• O34639 (AlphaFold)

Expression and Regulation

Operons

• Genes: snaA-tcyJ-tcyK-tcyL-tcyM-tcyN-cmoO-cmoI-cmoJ-ribR-sndA-ytnM

  Description: PubMed

  Regulation

  • induced in the presence of methionine and taurine PubMed

  Regulatory mechanism

  • CymR: repression, PubMed, in cymR regulon
  • AscR: activation, PubMed, in ascR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE29320 (ΔcmoI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATCACTCATTCTTCTGTCCC,  downstream forward: _UP4_GCACAGATAAAGGAGGCAAA
• BKK29320 (ΔcmoI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATCACTCATTCTTCTGTCCC,  downstream forward: _UP4_GCACAGATAAAGGAGGCAAA

References

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