nupN

168

lipoprotein, part of guanosine ABC transporter, binding protein

Locus: BSU_31540

Molecular weight: 37.19 kDa

Isoelectric point: 4.82

Protein length: 359 aa

Gene length: 1080 bp

Function: uptake of guanosine

Product: lipoprotein, part of guanosine transporter

Essential: no

Synonyms: nupN, yufN

Genomic Context

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List of homologs in different organisms, belongs to COG1744 (Galperin et al., 2021)

Gene

Coordinates: 3,239,930  3,241,009

Phenotypes of a mutant

• a ''nupN nupG'' double mutant is unable to utilize externally provided guanosine PubMed

The protein

Protein family

• BMP lipoprotein family (with Med, according to UniProt)

Structure

• 2FQW (PDB) (from Treponema pallidum, 45% identity) PubMed
• O05252 (AlphaFold)

Localization

• cell membrane (according to UniProt),  membrane associated PubMed

Expression and Regulation

Operons

• Genes: nupN-nupO-nupP-nupQ

  Description: PubMed

  Regulation

  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

  Regulatory mechanism

  • CodY: repression, PubMed, in codY regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-B553 (yufN::erm), available at the NBRP B. subtilis, Japan
• BKE31540 (nupN::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGATATAACCCCCCAGAA,  downstream forward: _UP4_TAAGAATCGCAGGGATAAGG
• BKK31540 (nupN::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGATATAACCCCCCAGAA,  downstream forward: _UP4_TAAGAATCGCAGGGATAAGG

References

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