HypO

• Description: NAD(P)H-flavin nitroreductase
  
  

Gene name	hypO
Synonyms	yfkO
Essential	no
Product	NAD(P)H-flavin nitroreductase
Function	protection against NaOCl stress
Gene expression levels in SubtiExpress: hypO
MW, pI	25 kDa, 5.667
Gene length, protein length	663 bp, 221 aa
Immediate neighbours	treR, yfkN
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

electron transport/ other, resistance against oxidative and electrophile stress

This gene is a member of the following regulons

HypR regulon

The gene

Basic information

• Locus tag: BSU07830

Phenotypes of a mutant

• a hypO ohrA mutant is more sensitive to NaOCl stress than ohrA single mutant PubMed

Database entries

• BsubCyc: BSU07830

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: nitroreductase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU07830

• Structure:

• UniProt: O34475

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: hypO PubMed

• Expression browser: hypO PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced under disulfide stress conditions (diamide, NaOCl) (HypR) PubMed

• Regulatory mechanism:
  • HypR:transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 292 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 638 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Haike Antelmann, University of Greifswald, Germany

References

Original articles

G A Prosser, A V Patterson, D F Ackerley
uvrB gene deletion enhances SOS chromotest sensitivity for nitroreductases that preferentially generate the 4-hydroxylamine metabolite of the anti-cancer prodrug CB1954.
J Biotechnol: 2010, 150(1);190-4
[PubMed:20727918] [WorldCat.org] [DOI] (I p)

Palm GJ, Khanh Chi B, Waack P, Gronau K, Becher D, Albrecht D, Hinrichs W, Read RJ, Antelmann H.
Structural insights into the redox-switch mechanism of the MarR/DUF24-type regulator HypR.
Nucleic Acids Res. 2012, Jan 11. [Epub ahead of print]
PubMed