glxB

168

glyoxalase II

Locus: BSU_32660

Molecular weight: 14.30 kDa

Isoelectric point: 4.61

Protein length: 127 aa

Gene length: 384 bp

Function: detoxification of methylglyoxal

Product: glyoxalase II

Essential: no

Synonyms: glxB, yurT

Genomic Context

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List of homologs in different organisms, belongs to COG0346 (Galperin et al., 2021)

Gene

Coordinates: 3,354,551  3,354,934

The protein

Catalyzed reaction/ biological activity

• S-lactoyl-bacillithiol --> D-lactate + bacillithiol PubMed

Protein family

• glyoxalase I family (with GlxA and YraH, according to UniProt)

Domains

• VOC domain (aa 1-127) (according to UniProt)

Structure

• 4G6X (PDB) (from Catenulispora acidiphila, 53% identity)
• O32161 (AlphaFold)

Expression and Regulation

Operons

• Genes: yuzN-glxB

  Description: PubMed

Biological materials

Mutant

• MGNA-B582 (yurT::erm), available at the NBRP B. subtilis, Japan
• BKE32660 (glxB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACTGTTTTTCCCTCCTT,  downstream forward: _UP4_TAACCGATTCAGTCGCGTCC
• BKK32660 (glxB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACTGTTTTTCCCTCCTT,  downstream forward: _UP4_TAACCGATTCAGTCGCGTCC

References

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