ycsA

168

tartrate dehydrogenase

Locus: BSU_04000

Molecular weight: 38.67 kDa

Isoelectric point: 5.11

Protein length: 354 aa

Gene length: 1065 bp

Function: utilization of tartrate

Product: tartrate dehydrogenase

Essential: no

E.C.: 1.1.1.93

Synonyms: ycsA

Genomic Context

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Categories containing this gene/protein

Metabolism, Carbon metabolism, Utilization of specific carbon sources, Utilization of organic acids

List of homologs in different organisms, belongs to COG0473 (Galperin et al., 2021)

This gene is a member of the following regulons

RoxS regulon

Gene

Coordinates: 452,830 → 453,894

The protein

Catalyzed reaction/ biological activity

L-tartrate + NAD⁺ --> oxaloglycolate + NADH PubMed
dihydroxyfumarate + NADH --> L-tartrate + NAD⁺ PubMed
meso-tartrate + NAD⁺ --> D-glycerate + CO₂ + NADH PubMed

Protein family

Isocitrate and isopropylmalate dehydrogenases family (with Icd and LeuB, according to UniProt)

Cofactors

Mn²⁺ PubMed

Structure

3FMX (PDB) (from Pseudomonas Putida , 51% identity)
P42958 (AlphaFold)

Paralogous protein(s)

LeuB, Icd

Expression and Regulation

Operons

Genes: ycsA

Description: PubMed

Expression

expression is reduced upon over expression of RoxS PubMed

Regulatory mechanism

RoxS: sRNA, PubMed, in roxS regulon

Biological materials

Mutant

MGNA-C020 (ycsA::erm), available at the NBRP B. subtilis, Japan
BKE04000 (ΔycsA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTCCTTCCCCTGTTAT,  downstream forward: _UP4_TGATGAATCAGGCCGGTGGC
BKK04000 (ΔycsA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTCCTTCCCCTGTTAT,  downstream forward: _UP4_TGATGAATCAGGCCGGTGGC

References

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