yvrC

168

ABC transporter (binding protein) for cobalamin

Locus: BSU_33180

Molecular weight: 34.08 kDa

Isoelectric point: 4.93

Protein length: 314 aa

Gene length: 945 bp

Function: uptake of cobalamin

Product: ABC transporter (binding protein)

Essential: no

Synonyms: yvrC

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0614 (Galperin et al., 2021)

Gene

Coordinates: 3,403,493 → 3,404,437

The protein

Protein family

• Bacterial solute-binding protein 8 family (according to UniProt)

Domains

• Fe/B12 periplasmic-binding domain (aa 59-311) (according to UniProt)

Structure

• 4M7O (PDB) (from Staphylococcus epidermidis, 45% identity)
• O34805 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: yvrC-yvrB-yvrA-yvqK

  Regulation

  • expression is decreased in the presence of cobalamin (vitamin B12) (B12 riboswitch) PubMed

  Regulatory mechanism

  • B12 riboswitch: RNA switch, upon binding of B12 PubMed, in B12 riboswitch

Biological materials

Mutant

• MGNA-B045 (yvrC::erm), available at the NBRP B. subtilis, Japan
• BKE33180 (ΔyvrC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATCTTTTCCTCCTAAA,  downstream forward: _UP4_CGTCTGATCGAAGGGGTCGA
• BKK33180 (ΔyvrC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATCTTTTCCTCCTAAA,  downstream forward: _UP4_CGTCTGATCGAAGGGGTCGA

References

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