yrvJ

168

secreted N-acetylmuramoyl-L-alanine amidase, contributes to septal peptidoglycan hydrolysis, required for efficient spore engulfment

Locus: BSU_27580

Molecular weight: 55.33 kDa

Isoelectric point: 5.19

Protein length: 518 aa

Gene length: 1557 bp

Function: cell wall metabolism

Product: peptidoglycan hydrolase

Essential: no

E.C.: 3.5.1.28

Synonyms: yrvJ

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG3103 (Galperin et al., 2021)

Gene

Coordinates: 2,818,494 → 2,820,050

The protein

Catalyzed reaction/ biological activity

• Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)

Protein family

• N-acetylmuramoyl-L-alanine amidase 3 family (according to UniProt)

Domains

• contains an amidase_3 domain (like CwlC, CwlD, LytC, YqiI)
• MurNAc-LAA domain (aa 346-514) (according to UniProt)
• 4 SH3b domains (aa 29-92, aa 102-164, aa 181-243, aa 258-320) (according to UniProt)

Structure

• O32041 (AlphaFold)

Expression and Regulation

Operons

• Genes: yrvJ

  Description: PubMed

Biological materials

Mutant

• BKE27580 (ΔyrvJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTGTCCTCCTTTCTGA,  downstream forward: _UP4_TAAAAAAAGCTGCCTTTTGG
• BKK27580 (ΔyrvJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGTGTCCTCCTTTCTGA,  downstream forward: _UP4_TAAAAAAAGCTGCCTTTTGG

References

Loading