yloN
168

tRNA adenosine methyltransferase

Locus

BSU_15750

Molecular weight

41.41 kDa

Isoelectric point

6.79

Protein length

363 aaSequenceBlast

Gene length

1092 bpSequenceBlast

Function

RNA modification

Product

tRNA methyltransferase

Essential

Synonyms

yloN, rlmN

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

Information processing, Protein synthesis, modification and degradation, Translation, rRNA modification and maturation/ based on similarity

Information processing, Protein synthesis, modification and degradation, Translation, tRNA modification and maturation

List of homologs in different organisms, belongs to COG0820 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon

Gene

Coordinates

1,649,286 1,650,377

The protein

Catalyzed reaction/ biological activity

methylates C-2 in A2503 in 23S rRNA and A37 in tRNA PubMed

adenosine²⁵⁰³ in 23S rRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine --> 2-methyladenosine²⁵⁰³ in 23S rRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine (according to UniProt)

adenosine³⁷ in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine --> 2-methyladenosine³⁷ in tRNA + 5'-deoxyadenosine + L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin] + S-adenosyl-L-homocysteine (according to UniProt)

Protein family

Radical SAM superfamily (according to UniProt)

Cofactors

Fe-S cluster PubMed

Structure

3RF9 (PDB) (from E. coli, 38% identity) PubMed

O34617 (AlphaFold)

Localization

cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

Genes