yjbO

yjbO
168

pseudouridylate synthase, pseudouridylation of position 31 (and also 32) in several tRNAs

Locus
BSU_11620
Molecular weight
31.32 kDa
Isoelectric point
6.55
Protein length
Gene length
Function
RNA modification
Product
pseudouridylate synthase
Essential
no
Synonyms
yjbO

Genomic Context

List of homologs in different organisms, belongs to COG0564 (Galperin et al., 2021)

Gene
Coordinates
1,238,523  1,239,374
The protein
Catalyzed reaction/ biological activity
uridine in RNA --> ψ-uridine at position 31 in several tRNAs PubMed
Protein family
S4 RNA-binding domain superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
pseudouridine synthase RluA family (with YhcT and RluD, according to UniProt)
S4 RNA-binding domain (aa 25-79) (according to UniProt)
Structure
1V9F (PDB) (RluD from E. coli, 40% identical, aa 64 - 279) PubMed
Paralogous protein(s)
Expression and Regulation
Operons
Description
Regulation
expressed during exponential growth
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yjbLyjbO

2025-07-22 20:25:09

Jstuelk

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c5edd61e714281007d4d9ec99a1efa0cfef63f09

83E46551CBC32AB07130281546FE3D422612C2C3

Biological materials
Mutant
MGNA-B161 (yjbO::erm), available at the NBRP B. subtilis, Japan
BKE11620 (yjbO::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATCCCGTCCTCAGCGGAAG,  downstream forward: _UP4_GAGAATCATTGATTCTCTCC
BKK11620 (yjbO::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATCCCGTCCTCAGCGGAAG,  downstream forward: _UP4_GAGAATCATTGATTCTCTCC
References
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Time of last update: 2025-07-29 15:33:04

Author of last update: Jstuelk