yjbO

168

pseudouridylate synthase, pseudouridylation of position 31 (and also 32) in several tRNAs

Locus: BSU_11620

Molecular weight: 31.32 kDa

Isoelectric point: 6.55

Protein length: 283 aa

Gene length: 852 bp

Function: RNA modification

Product: pseudouridylate synthase

Essential: no

Synonyms: yjbO

Genomic Context

Hide small RNAsZoom:

Categories containing this gene/protein

Information processing, Protein synthesis, modification and degradation, Translation, tRNA modification and maturation

List of homologs in different organisms, belongs to COG0564 (Galperin et al., 2021)

Gene

Coordinates: 1,238,523  1,239,374

The protein

Catalyzed reaction/ biological activity

uridine in RNA --> ψ-uridine at position 31 in several tRNAs PubMed

Protein family

S4 RNA-binding domain superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
pseudouridine synthase RluA family (with YhcT and RluD, according to UniProt)

Domains

S4 RNA-binding domain (aa 25-79) (according to UniProt)

Structure

1V9F (PDB) (RluD from E. coli, 40% identical, aa 64 - 279) PubMed
O31613 (AlphaFold)

Paralogous protein(s)

RluD, YhcT

Expression and Regulation

Operons

Genes: yjbL-sasB-nadF-yjbO

Description: PubMed

Regulation

expressed during exponential growth

Biological materials

Mutant

MGNA-B161 (yjbO::erm), available at the NBRP B. subtilis, Japan
BKE11620 (yjbO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCCGTCCTCAGCGGAAG,  downstream forward: _UP4_GAGAATCATTGATTCTCTCC
BKK11620 (yjbO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCCCGTCCTCAGCGGAAG,  downstream forward: _UP4_GAGAATCATTGATTCTCTCC

References

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