yjbO

168

pseudouridylate synthase, pseudouridylation of position 31 (and also 32) in several tRNAs

locus: BSU_11620

Molecular weight: 31.32 kDa

pI: 6.55

Protein length: 283 aa

Gene length: 852 bp

function: RNA modification

product: pseudouridylate synthase

essential: no

synonyms: yjbO

List of homologs in different organisms, belongs to COG0564 (Galperin et al., 2021)

Gene

Coordinates: 1,238,523  1,239,374

The protein

Catalyzed reaction/ biological activity

• uridine in RNA --> ψ-uridine at position 31 in several tRNAs [pubmed|32629984]

Protein family

• [wiki|S4 RNA-binding domain] superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
• pseudouridine synthase RluA family (with [protein|064AE8CE6D52E35582F90654AA9548963573C05C|yhcT] and [protein|C8C2E2F1B739F3D9A2FA6B460998708AAB2DA495|rluD], according to UniProt)

[wiki|Domains]

• [wiki|S4 RNA-binding domain] (aa 25-79) (according to UniProt)

Structure

• [PDB|1V9F] (RluD from E. coli, 40% identical, aa 64 - 279) [pubmed|15078091]
• [AF|O31613]

Paralogous protein(s)

• [protein|C8C2E2F1B739F3D9A2FA6B460998708AAB2DA495|rluD], [protein|064AE8CE6D52E35582F90654AA9548963573C05C|yhcT]

Expression and Regulation

Operons

• genes: [gene|E5A57660B69F9E9BC52FD9C8A5E3C8786CE96728|yjbL]-[gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB]-[gene|2A2640A0501ECEA44A3C0C4842FAC053FB422A1F|nadF]-[gene|2AECDA9C4D0E704976B9E2168722EC80FE7A256D|yjbO]

  description: [Pubmed|18067544]

  regulation

  • expressed during exponential growth

Biological materials

Mutant

• MGNA-B161 (yjbO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1160 NBRP B. subtilis, Japan]
• BKE11620 ([gene|2AECDA9C4D0E704976B9E2168722EC80FE7A256D|yjbO]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11620 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCCGTCCTCAGCGGAAG,  downstream forward: _UP4_GAGAATCATTGATTCTCTCC
• BKK11620 ([gene|2AECDA9C4D0E704976B9E2168722EC80FE7A256D|yjbO]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11620 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCCCGTCCTCAGCGGAAG,  downstream forward: _UP4_GAGAATCATTGATTCTCTCC

References

• 18067544,15078091,32629984