uppS

168

undecaprenyl pyrophosphate synthetase, provides the precursor for the carrier lipid, undecaprenyl phosphate (C55-P), which is required for cell wall synthesis

Locus: BSU_16530

Molecular weight: 29.63 kDa

Isoelectric point: 5.41

Protein length: 260 aa

Gene length: 783 bp

Function: cell wall biosynthesis

Product: undecaprenyl pyrophosphate synthetase

Essential: yes

E.C.: 2.5.1.31

Synonyms: uppS, yluA

Genomic Context

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List of homologs in different organisms, belongs to COG0020 (Galperin et al., 2021)

Gene

Coordinates: 1,721,214  1,721,996

Phenotypes of a mutant

• essential PubMed
• uppS is dispensable in wall-less L-forms PubMed
• the uppS86 (C257T) mutation provides resistance to rare earth metals such as scandium and to bacitracin PubMed
• inactivation suppresses the essentiality of mbl PubMed

The protein

Catalyzed reaction/ biological activity

• catalyzes eight consecutive condensation reactions of isopentenyl pyrophosphate (IPP) with farnesyl pyrophosphate (FPP) to form undecaprenyl pyrophosphate

Protein family

• UPP synthetase family (single member, according to UniProt)

Structure

• 1X09 (PDB) (the protein from E. coli, 49% identity, 77% similarity) PubMed
• O31751 (AlphaFold)

Expression and Regulation

Operons

• Genes: uppS-cdsA-ispC-rasP-proS-polC

  Description: PubMed

Biological materials

Mutant

• MGNA-B080 (yluA::erm), available at the NBRP B. subtilis, Japan

References

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