trmK

168

tRNA:m1A22 methyl transferase

Locus: BSU_25180

Molecular weight: 23.56 kDa

Isoelectric point: 5.27

Protein length: 216 aa

Gene length: 651 bp

Function: tRNA modification

Product: tRNA:m1A22 methyl transferase

Essential: no

Synonyms: trmK, yqfN

Genomic Context

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List of homologs in different organisms, belongs to COG2384 (Galperin et al., 2021)

Gene

Coordinates: 2,598,616 → 2,599,266

The protein

Catalyzed reaction/ biological activity

• Catalyzes the S-adenosyl-L-methionine-dependent formation of N₁-methyladenine at position 22 (m1A22) in tRNA PubMed
• adenosine²² in tRNA + S-adenosyl-L-methionine --> H⁺ + N¹-methyladenosine²² in tRNA + S-adenosyl-L-homocysteine PubMed

Protein family

• TrmK family (single member, according to UniProt)

Domains

• N-terminal Class I MTase domain PubMed
• C-terminal coiled-coil domain PubMed

Cofactors

• SAM PubMed

Structure

• 6Q56 (PDB) PubMed
• P54471 (AlphaFold)

Modification

• phosphorylation on Ser-48 PubMed

Localization

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• Genes: trmK-yqfO

  Description

  • PubMed

Biological materials

Mutant

• BKE25180 (ΔtrmK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCACCGCTCCATTCGGTA,  downstream forward: _UP4_GATCATGGCTAAAAGTGTAA
• BKK25180 (ΔtrmK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCACCGCTCCATTCGGTA,  downstream forward: _UP4_GATCATGGCTAAAAGTGTAA

References

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