sspB

168

small acid-soluble spore protein (major beta-type SASP)

Locus: BSU_09750

Molecular weight: 6.84 kDa

Isoelectric point: 4.79

Protein length: 67 aa

Gene length: 204 bp

Function: protection of spore DNA

Product: small acid-soluble spore protein (major beta-type SASP)

Essential: no

Synonyms: sspB

Genomic Context

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List of homologs in different organisms, belongs to COG5852 (Galperin et al., 2021)

Gene

Coordinates: 1,050,031  1,050,234

Phenotypes of a mutant

• a ''sspB'' mutant is sensitive to ionizing radiation PubMed
• a sspA sspB double mutant is sensitive to blue light-induced DNA damage PubMed

The protein

Protein family

• Alpha/beta-type SASP family (according to UniProt)

Structure

• 2Z3X (PDB) (SspC in complex with DNA, 78% identity)
• P04832 (AlphaFold)

Modification

• phosphorylated on Ser-6, Ser-7, and Ser-45 PubMed
• phosphorylated on Arg-65 PubMed

Paralogous protein(s)

• SspA, SspC, SspD

Expression and Regulation

Operons

• Genes: sspB

  Description: PubMed

  Regulation

  • expressed during sporulation in the forespore (SigG, SpoVT) PubMed

  Regulatory mechanism

  • SpoVT: activation, PubMed, in spoVT regulon

  Sigma factors

  • SigG: sigma factor, PubMed, in sigG regulon

  Additional information

  • the mRNA half-life is about 13 min PubMed

Additional information

• the mRNA belongs to the 46 most abundant mRNA species in dormant spores PubMed

Biological materials

Mutant

• 1S111 (no resistance), PubMed, available at BGSC
• 1S113 (no resistance), PubMed, available at BGSC
• BKE09750 (sspB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTGTAAAATCTCCTTTT,  downstream forward: _UP4_TAACAATTCAATATATGGCT
• BKK09750 (sspB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTGTAAAATCTCCTTTT,  downstream forward: _UP4_TAACAATTCAATATATGGCT

GFP fusion

• GP996 (cat, based on pSG1151), available in Jörg Stülke's lab

References

Reviews

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Original Publications

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