spoVIF

168

required for spore coat assembly and resistance

Locus: BSU_11810

Molecular weight: 11.45 kDa

Isoelectric point: 10.2

Protein length: 84 aa

Gene length: 255 bp

Function: required for spore coat assembly and resistance

Product: unknown

Essential: no

E.C.: null

Synonyms: spoVIF, yjcC

Genomic Context

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List of homologs in different organisms, belongs to COG5847 (Galperin et al., 2021)

Gene

Coordinates: 1,253,385  1,253,639

The protein

Structure

• O31625 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: yjzK-yjcZ-spoVIF

  Description: PubMed

• Genes: spoVIF

  Description: PubMed

  Regulation

  • expressed late during sporulation in the mother cell (SigK, GerR) PubMed

  Regulatory mechanism

  • GerR: activation, PubMed, in gerR regulon

  Sigma factors

  • SigK: sigma factor, PubMed, in sigK regulon

Biological materials

Mutant

• MGNA-B295 (yjcC::erm), available at the NBRP B. subtilis, Japan
• BKE11810 (spoVIF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATGCGCCTCCTTTTGG,  downstream forward: _UP4_TAAGTGGATGGCCTGGCTGA
• BKK11810 (spoVIF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAATGCGCCTCCTTTTGG,  downstream forward: _UP4_TAAGTGGATGGCCTGGCTGA

References

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