rsbRB

168

part of the stressosome

Locus: BSU_13200

Molecular weight: 32.23 kDa

Isoelectric point: 4.79

Protein length: 277 aa

Gene length: 834 bp

Function: control of SigB activity

Product: RsbR paralog

Essential: no

Synonyms: rsbRB, ispU, ykoB

Genomic Context

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List of homologs in different organisms, belongs to COG1366 (Galperin et al., 2021)

Gene

Coordinates: 1,387,206 → 1,388,039

The protein

Catalyzed reaction/ biological activity

• negative regulator of YtvA-dependent light activation of the SigB stress response PubMed

Domains

• STAS domain (aa 165-276) (according to UniProt)

Structure

• 2MWG (PDB) (YtvA, corresponds to aa 161 ... 273, 34% identity)
• O34860 (AlphaFold)

Modification

• phosphorylation on Thr-186 and probably also on Thr-220 by RsbT PubMed

Paralogous protein(s)

• RsbRC, RsbR, YtvA, RsbRD

Expression and Regulation

Operons

• Genes: rsbRB

  Description: PubMed

Biological materials

Mutant

• BKE13200 (ΔrsbRB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGACACTGCTCCTTTCCC,  downstream forward: _UP4_TAAAAAAATCCGCTATCTGT
• BKK13200 (ΔrsbRB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGACACTGCTCCTTTCCC,  downstream forward: _UP4_TAAAAAAATCCGCTATCTGT

References

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