rbsK

168

ribokinase

Locus: BSU_35920

Molecular weight: 30.99 kDa

Isoelectric point: 4.61

Protein length: 293 aa

Gene length: 882 bp

Function: ribose utilization

Product: ribokinase

Essential: no

E.C.: 2.7.1.15

Synonyms: rbsK

Genomic Context

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Categories containing this gene/protein

Metabolism, Carbon metabolism, Utilization of specific carbon sources, Utilization of ribose

List of homologs in different organisms, belongs to COG0524 (Galperin et al., 2021)

This gene is a member of the following regulons

SigA regulon, CcpA regulon, AbrB regulon

Gene

Coordinates: 3,702,393 → 3,703,274

The protein

Catalyzed reaction/ biological activity

ATP + D-ribose --> ADP + D-ribose 5-phosphate + H⁺ (according to UniProt)

Protein family

carbohydrate kinase PfkB family (according to UniProt)

Structure

1VM7 (PDB) (the enzyme of Thermotoga maritima, 38% identity)
P36945 (AlphaFold)

Localization

cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

Genes: rbsR-rbsK-rbsD-rbsA-rbsC-rbsB

Description: PubMed

Regulation

expressed upon transition into the stationary phase (AbrB) PubMed

Regulatory mechanism

CcpA: repression, PubMed, in ccpA regulon
AbrB: activation, PubMed, in abrB regulon

Sigma factors

SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

BKE35920 (ΔrbsK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ACCAATCACACAAATATTAC,  downstream forward: _UP4_AATGAAGTAGAGGAGCTGCT
BKK35920 (ΔrbsK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ACCAATCACACAAATATTAC,  downstream forward: _UP4_AATGAAGTAGAGGAGCTGCT

References

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