pyrB

168

aspartate carbamoyltransferase

Locus: BSU_15490

Molecular weight: 34.02 kDa

Isoelectric point: 5.34

Protein length: 304 aa

Gene length: 915 bp

Function: pyrimidine biosynthesis

Product: aspartate carbamoyltransferase

Essential: no

E.C.: 2.1.3.2

Synonyms: pyrB

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0540 (Galperin et al., 2021)

Gene

Coordinates: 1,620,476 → 1,621,390

The protein

Catalyzed reaction/ biological activity

• carbamoyl-P + L-aspartate --> H⁺ + N-carbamoyl-L-aspartate + phosphate (according to UniProt)

Protein family

• ATCase/OTCase family (with ArgF, according to UniProt)

Structure

• 3R7D (PDB) PubMed
• P05654 (AlphaFold)

Modification

• phosphorylation on Ser-303 PubMed

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and Regulation

Operons

• Genes: pyrR-pyrP-pyrB-pyrC-pyrAA-pyrAB-pyrK-pyrD-pyrF-pyrE

  Description: PubMed

  Regulation

  • induced in the absence of uridine nucleotides (PyrR) PubMed

  Regulatory mechanism

  • PyrR: termination, via RNA switch, in pyrR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Additional information

• expression is reduced in motile cells as compared to non-motile cells PubMed

Biological materials

Mutant

• BKE15490 (ΔpyrB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTCTTTCCCCTCTCTT,  downstream forward: _UP4_AATGTGAAAAGAGGAGAAGC
• BKK15490 (ΔpyrB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTCTTTCCCCTCTCTT,  downstream forward: _UP4_AATGTGAAAAGAGGAGAAGC

References

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