nprB

168

extracellular neutral protease B, required for the function of the YitM toxin

Locus: BSU_11100

Molecular weight: 59.17 kDa

Isoelectric point: 5.32

Protein length: 538 aa

Gene length: 1617 bp

Function: protection of B. subtilis biofilms against competitors

Product: extracellular neutral protease B

Essential: no

Synonyms: nprB

Genomic Context

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List of homologs in different organisms, belongs to COG3227 (Galperin et al., 2021)

Gene

Coordinates: 1,186,037  1,187,653

The protein

Protein family

• peptidase M4 family (with NprE, according to UniProt)

Structure

• 5A3Y (PDB) (thermolysin, 44% identity) PubMed
• P39899 (AlphaFold)

Paralogous protein(s)

• NprE

Localization

• secreted (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: nprB

  Description: PubMed

  Regulatory mechanism

  • DegU: activation, PubMed, in degU regulon

Biological materials

Mutant

• KO7 (nprE  aprE  epr  mpr  nprB  vpr  bpr), available as BGSC 1A1133
• BKE11100 (nprB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAACACCACATCCTTCCT,  downstream forward: _UP4_TGAGCAAACAAAAACAGTCA
• BKK11100 (nprB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAACACCACATCCTTCCT,  downstream forward: _UP4_TGAGCAAACAAAAACAGTCA

References

Reviews

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Original Publications

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