moaE

168

molybdopterin synthase (large subunit), catalyses the transfer of sulfide from MoaD thiocarboxylate

Locus: BSU_14300

Molecular weight: 17.64 kDa

Isoelectric point: 4.75

Protein length: 157 aa

Gene length: 474 bp

Function: nitrate respiration

Product: molybdopterin synthase (large subunit)

Essential: no

E.C.: null

Synonyms: moaE

Genomic Context

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List of homologs in different organisms, belongs to COG0314 (Galperin et al., 2021)

Gene

Coordinates: 1,498,966  1,499,439

The protein

Catalyzed reaction/ biological activity

• catalyses the transfer of sulfide from MoaD thiocarboxylate
• 2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-NH-CH₂-C(O)SH + cyclic pyranopterin phosphate + H₂O --> 2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + 4 H⁺ + molybdopterin (according to UniProt)

Protein family

• moaE family (single member, according to UniProt)

Structure

• 1NVI (PDB) (the MoaD-MoaE complex from E. coli)  PubMed
• O31705 (AlphaFold)

Expression and Regulation

Operons

• Genes: mobA-moeB-moeA-mobB-moaE-moaD

  Description: PubMed

Biological materials

Mutant

• BKE14300 (moaE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTCGTAATCTCAAACCGTT,  downstream forward: _UP4_CCAGACCTAAGCGAGGGAGA
• BKK14300 (moaE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTCGTAATCTCAAACCGTT,  downstream forward: _UP4_CCAGACCTAAGCGAGGGAGA

References

Reviews

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Original Publications

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