menH

168

menaquinone biosynthesis methyltransferase

Locus: BSU_22750

Molecular weight: 26.43 kDa

Isoelectric point: 8.17

Protein length: 233 aa

Gene length: 702 bp

Function: biosynthesis of menaquinone

Product: menaquinone biosynthesis methyltransferase

Essential: no

E.C.: 2.1.1.163

Synonyms: menH, gerCB, menG

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2226 (Galperin et al., 2021)

Gene

Coordinates: 2,382,907 → 2,383,608

Phenotypes of a mutant

• essential according to Kobayashi et al.; however, inactivation reported by Meeske et al. and Block et al.
• inactivation of ''menH'' reduces sporulation efficiency to 12.7% that of wild type cells PubMed
• defective in biofilm formation PubMed

The protein

Catalyzed reaction/ biological activity

• 2-demethylmenaquinol + S-adenosyl-L-methionine --> menaquinol + H⁺ + S-adenosyl-L-homocysteine (according to UniProt)

Protein family

• Methyltransferase superfamily (according to UniProt)
• class I-like SAM-binding methyltransferase superfamily (according to UniProt)

Domains

• AB hydrolase-1 domain (aa 26-259) (according to InterPro)

Structure

• 1OBW (PDB) (from Saccharomyces cerevisiae, 31% identity) PubMed
• P31113 (AlphaFold)

Expression and Regulation

Operons

• Genes: hepS-menH-hepT-ndk

  Description: PubMed

Additional information

• highly expressed at high iron concentrations PubMed

Biological materials

Mutant

• BKE22750 (ΔmenH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCTTTTGAGTCCTGCATAA,  downstream forward: _UP4_CTGTTTGAAGAGGCGGGCCT
• BKK22750 (ΔmenH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTCTTTTGAGTCCTGCATAA,  downstream forward: _UP4_CTGTTTGAAGAGGCGGGCCT

References

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