menF

168

menaquinone-specific isochorismate synthase

Locus: BSU_30830

Molecular weight: 52.65 kDa

Isoelectric point: 4.71

Protein length: 471 aa

Gene length: 1416 bp

Function: biosynthesis of menaquinone

Product: menaquinone-specific isochorismate synthase

Essential: no

E.C.: 5.4.4.2

Synonyms: menF, icsM, entC

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1169 (Galperin et al., 2021)

Gene

Coordinates: 3,152,302  3,153,717

The protein

Catalyzed reaction/ biological activity

• chorismate --> isochorismate (according to UniProt)

Protein family

• isochorismate synthase family (with DhbC, according to UniProt)

Structure

• 3BZM (PDB) (from Escherichia coli, 33% identity, 51% similarity) PubMed
• P23973 (AlphaFold)

Expression and Regulation

Operons

• Genes: menF-menD-ytxM-menB-menE-menC

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • A ncRNA is predicted between 'mntA' and 'menC' PubMed

Biological materials

Mutant

• BKE30830 (menF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAGACATTCCTCCATAA,  downstream forward: _UP4_TCTGCATTAGGAGGTGAGAG
• BKK30830 (menF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAGACATTCCTCCATAA,  downstream forward: _UP4_TCTGCATTAGGAGGTGAGAG

References

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