iolD

168

3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase, formation of 5-deoxy-D-glucuronic acid (3rd reaction)

Locus: BSU_39730

Molecular weight: 63.96 kDa

Isoelectric point: 5.01

Protein length: 637 aa

Gene length: 1914 bp

Function: myo-inositol catabolism

Product: 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase

Essential: no

Synonyms: iolD, yxdD

Genomic Context

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List of homologs in different organisms, belongs to COG3962 (Galperin et al., 2021)

Gene

Coordinates: 4,079,083  4,080,996

The protein

Catalyzed reaction/ biological activity

• 3D-3,5/4-trihydroxycyclohexane-1,2-dione + H₂O --> 5-deoxy-D-glucuronate + H⁺ (according to UniProt)

Protein family

• TPP enzyme family (according to UniProt)

Cofactors

• TPP (according to UniProt)

Structure

• 1V5E (PDB) (pyruvate oxidase from Aerococcus viridans, 25% identity)
• P42415 (AlphaFold)

Expression and Regulation

Operons

• Genes: iolA-iolB-iolC-iolD-iolE-iolF-iolG-iolH-iolI-iolJ

  Description: PubMed

  Regulation

  • induced by inositol (IolR) PubMed

  Regulatory mechanism

  • IolR: repression, PubMed, in iolR regulon
  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-B773 (iolD::erm), available at the NBRP B. subtilis, Japan
• BKE39730 (iolD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAAACGATCCCACCTTTA,  downstream forward: _UP4_CAGTATTAGAGGGGAGGCAC
• BKK39730 (iolD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAAACGATCCCACCTTTA,  downstream forward: _UP4_CAGTATTAGAGGGGAGGCAC

References

Reviews

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Original Publications

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Labs working on this gene/protein

• Ken-ichi Yoshida, Kobe University, Japan