glpT

168

glycerol-3-phosphate permease

Locus: BSU_02140

Molecular weight: 49.64 kDa

Isoelectric point: 9.37

Protein length: 444 aa

Gene length: 1335 bp

Function: glycerol-3-phosphate uptake

Product: glycerol-3-phosphate permease

Essential: no

Synonyms: glpT, ybeE

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2271 (Galperin et al., 2021)

Gene

Coordinates: 233,994 → 235,328

The protein

Catalyzed reaction/ biological activity

• uptake of glycerol-3-phosphate

Protein family

• major facilitator superfamily (according to UniProt)

Structure

• 1PW4 (PDB) (from E. coli, 60% identity) PubMed
• P37948 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: glpT-glpQ

  Description: PubMed

  Regulation

  • induced by glycerol (GlpP) PubMed

  Regulatory mechanism

  • GlpP: antitermination, via a protein-dependent RNA switch PubMed, in glpP regulon
  • PhoP: activation, PubMed, in phoP regulon
  • CcpA: repression, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Additional information

• increased expression at increased temperature (via an RNA thermometer that covers the Ribosomal binding site of the mRNA) PubMed

Biological materials

Mutant

• QB5437 (ermC), available in the Stülke lab
• BKE02140 (ΔglpT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAATAATTATCCCCCTT,  downstream forward: _UP4_TAAGAAAGACACATAAAAGA
• BKK02140 (ΔglpT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTAATAATTATCCCCCTT,  downstream forward: _UP4_TAAGAAAGACACATAAAAGA

References

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