fadE

168

acyl-CoA dehydrogenase

Locus: BSU_32820

Molecular weight: 65.16 kDa

Isoelectric point: 5.15

Protein length: 594 aa

Gene length: 1785 bp

Function: fatty acid degradation

Product: acyl-CoA dehydrogenase

Essential: no

Synonyms: fadE, yusJ

Genomic Context

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List of homologs in different organisms, belongs to COG1960 (Galperin et al., 2021)

Gene

Coordinates: 3,367,040 → 3,368,824

The protein

Catalyzed reaction/ biological activity

• A + 2,3-saturated acyl-CoA --> 2,3-dehydroacyl-CoA + AH₂ (according to UniProt)

Protein family

• Acyl-CoA dehydrogenase family (according to UniProt)

Cofactors

• FAD (according to UniProt)

Structure

• 2Z1Q (PDB) (from Thermus thermophilus hb8, 52% identity, 68% similarity)
• O32176 (AlphaFold)

Paralogous protein(s)

• YngJ, AcdA
• MmgC, Array

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: fadN-fadA-fadE

  Description: PubMed

  Regulation

  • ''fadN'': induced by long-chain fatty acids (FadR) PubMed

  Regulatory mechanism

  • SdpR: activation, PubMed, in sdpR regulon
  • FadR: repression, PubMed, in fadR regulon
  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: fadM-fadN-fadA-fadE

  Description: PubMed

  Regulation

  • ''fadM'': induced by long-chain fatty acids (FadR) PubMed

  Regulatory mechanism

  • FadR: repression, PubMed, in fadR regulon
  • SdpR: activation, PubMed, in sdpR regulon

Biological materials

Mutant

• MGNA-A602 (yusJ::erm), available at the NBRP B. subtilis, Japan
• BKE32820 (''fadE::erm'', available in the BGSC, in Fabian Commichau's, and in Jörg Stülke's lab) PubMed
• BKE32820 (ΔfadE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGTTTTTCCCCCTTTA,  downstream forward: _UP4_TGATTGGCCATGAGCTCCGC
• BKK32820 (ΔfadE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGTTTTTCCCCCTTTA,  downstream forward: _UP4_TGATTGGCCATGAGCTCCGC

References

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