yxaL

168

similar to Ser/Thr kinase, increases the processivity of the PcrA helicase

Locus: BSU_39940

Molecular weight: 43.72 kDa

Isoelectric point: 6.38

Protein length: 410 aa

Gene length: 1233 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yxaL, yxaK

Genomic Context

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List of homologs in different organisms, belongs to COG1520 (Galperin et al., 2021)

Gene

Coordinates: 4,102,429  4,103,661

The protein

Structure

• 3HXJ (PDB) (from Methanococcus maripaludis, 33% Identity)
• P42111 (AlphaFold)

Localization

• extracellular (signal peptide), major constituent of the secretome PubMed

Expression and Regulation

Operons

• Genes: yxaL

  Description: PubMed

• Genes: yxaJ-yxaL

  Description: PubMed

  Regulation

  • repressed by Rok PubMed

  Regulatory mechanism

  • Rok: repression, PubMed, in rok regulon
  • DnaA: repression, Rok-DnaA PubMed, in dnaA regulon
  • AbrB: repression, PubMed, in abrB regulon

Biological materials

Mutant

• MGNA-B687 (yxaL::erm), available at the NBRP B. subtilis, Japan
• BKE39940 (yxaL::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCATTGAGACCCCCATTA,  downstream forward: _UP4_TAACTGAATAGAATAGCAGA
• BKK39940 (yxaL::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCATTGAGACCCCCATTA,  downstream forward: _UP4_TAACTGAATAGAATAGCAGA

Expression vectors

• pGP392 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Jörg Stülke's lab
• for expression, purification in E. coli with N-terminal Strep-tag, in pGP172: pGP824, available in Jörg Stülke's lab, PubMed

References

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