phoC

168

HAD superfamily sugar phosphate phosphatase, dephosphorylation of N-acetyl-glucosamine 6-phosphate

Locus: BSU_36290

Molecular weight: 31.80 kDa

Isoelectric point: 4.88

Protein length: 285 aa

Gene length: 858 bp

Function: detoxification of sugar phosphates

Product: sugar phosphate phosphatase

Essential: no

Synonyms: phoC, ywpJ

Genomic Context

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List of homologs in different organisms, belongs to COG0561 (Galperin et al., 2021)

Gene

Coordinates: 3,738,343 → 3,739,200

The protein

Catalyzed reaction/ biological activity

• dephosphorylates glycerol 3-phosphate and ribose 5- phosphate PubMed
• dephosphorylation of N-acetyl-glucosamine 6-phosphate PubMed

Protein family

• HAD superfamily (according to UniProt)
• Cof family (according to UniProt)

Cofactors

• Mg²⁺ PubMed

Structure

• 1NRW (PDB)
• P94592 (AlphaFold)

Modification

• phosphorylated on Arg-258 PubMed

Expression and Regulation

Operons

• Genes: glcR-phoC

  Description: PubMed

  Regulation

  • induced by phosphosugar stress PubMed

  Regulatory mechanism

  • GlcR: repression, PubMed, in glcR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A531 (ywpJ::erm), available at the NBRP B. subtilis, Japan
• BKE36290 (ΔphoC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATCGCAATTAATTTCACGT,  downstream forward: _UP4_TAAGTATATGTGCTGCCACA
• BKK36290 (ΔphoC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATCGCAATTAATTTCACGT,  downstream forward: _UP4_TAAGTATATGTGCTGCCACA

References

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