ytxK

168

similar to SAM-dependent methyltransferase

locus: BSU_29480

Molecular weight: 37.20 kDa

pI: 5.41

Protein length: 329 aa

Gene length: 990 bp

function: DNA modification

product: similar to SAM-dependent DNA-methyltransferase

essential: no

synonyms: ytxK, ythI

List of homologs in different organisms, belongs to COG0827 (Galperin et al., 2021)

Gene

Coordinates: 3,016,646  3,017,635

The protein

Structure

• [PDB|2F8L] (from Listeria monocytogenes, 45% identity)
• [AF|P37876]

Expression and Regulation

Operons

• genes: [gene|2501228D435B151F151A3C0EF10DE99A8395977F|tpx]-[gene|2C83EECFA809F34ADCA6445CF2E6BAC730D523DB|ytxK]

  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [pubmed|12642660,19575568], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]

• genes: [gene|2C83EECFA809F34ADCA6445CF2E6BAC730D523DB|ytxK]

  description: [Pubmed|8226682]

Biological materials

Mutant

• MGNA-A114 (ytxK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/114 NBRP B. subtilis, Japan]
• BKE29480 ([gene|2C83EECFA809F34ADCA6445CF2E6BAC730D523DB|ytxK]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29480 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTGTGTTCCTCCTTGC,  downstream forward: _UP4_TAAGTGTTTTAAAAGCAGCT
• BKK29480 ([gene|2C83EECFA809F34ADCA6445CF2E6BAC730D523DB|ytxK]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29480 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTGTGTTCCTCCTTGC,  downstream forward: _UP4_TAAGTGTTTTAAAAGCAGCT

References

• 8226682