ytxK

168

similar to SAM-dependent methyltransferase

Locus: BSU_29480

Molecular weight: 37.20 kDa

Isoelectric point: 5.41

Protein length: 329 aa

Gene length: 990 bp

Function: DNA modification

Product: similar to SAM-dependent DNA-methyltransferase

Essential: no

Synonyms: ytxK, ythI

Genomic Context

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List of homologs in different organisms, belongs to COG0827 (Galperin et al., 2021)

Gene

Coordinates: 3,016,646  3,017,635

The protein

Structure

• 2F8L (PDB) (from Listeria monocytogenes, 45% identity)
• P37876 (AlphaFold)

Expression and Regulation

Operons

• Genes: tpx-ytxK

  Description: PubMed

  Regulatory mechanism

  • Spx: activation, PubMed, in spx regulon

• Genes: ytxK

  Description: PubMed

Biological materials

Mutant

• MGNA-A114 (ytxK::erm), available at the NBRP B. subtilis, Japan
• BKE29480 (ytxK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTGTGTTCCTCCTTGC,  downstream forward: _UP4_TAAGTGTTTTAAAAGCAGCT
• BKK29480 (ytxK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTGTGTTCCTCCTTGC,  downstream forward: _UP4_TAAGTGTTTTAAAAGCAGCT

References

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