ytcJ

168

putative amidohydrolase

Locus: BSU_29550

Molecular weight: 58.31 kDa

Isoelectric point: 5.46

Protein length: 529 aa

Gene length: 1590 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: ytcJ

Genomic Context

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List of homologs in different organisms, belongs to COG1574 (Galperin et al., 2021)

Gene

Coordinates: 3,022,068 → 3,023,657

The protein

Protein family

• Metallo-dependent hydrolases superfamily (according to UniProt)

Structure

• 3IGH (PDB) (from Pyrococcus horikoshii, 29% identity)
• O34355 (AlphaFold)

Expression and Regulation

Operons

• Genes: ytcI-ytcJ

  Description: PubMed

Biological materials

Mutant

• MGNA-A108 (ytcJ::erm), available at the NBRP B. subtilis, Japan
• BKE29550 (ΔytcJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTTCATTCACGTTCACTCTC,  downstream forward: _UP4_TAAAAGGACAGGCAGCAGCC
• BKK29550 (ΔytcJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CTTCATTCACGTTCACTCTC,  downstream forward: _UP4_TAAAAGGACAGGCAGCAGCC

References

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