reoY

168

effector protein of MurAA degradation by ClpC-ClpP

Locus: BSU_22580

Molecular weight: 21.22 kDa

Isoelectric point: 5.88

Protein length: 179 aa

Gene length: 540 bp

Function: control of peptidoglycan biosynthesis

Product: regulator of MurAA degradation

Essential: no

Synonyms: reoY, ypiB

Genomic Context

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List of homologs in different organisms, belongs to COG5582 (Galperin et al., 2021)

Gene

Coordinates: 2,365,736 → 2,366,275

Phenotypes of a mutant

• accumulation of MurAA due to reduced degradation by ClpC-ClpP PubMed

The protein

Protein family

• UPF0302 family (single member, according to UniProt)

Structure

• 3DO9 (PDB) (from B. anthracis, 64% identity)
• P54390 (AlphaFold)

Expression and Regulation

Operons

• Genes: ypiA-reoY

  Description: PubMed

• Genes: reoY

  Description: PubMed

Biological materials

Mutant

• MGNA-A408 (ypiB::erm), available at the NBRP B. subtilis, Japan
• BKE22580 (ΔreoY::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTAATTCCCTCCTCTAT,  downstream forward: _UP4_TAATATGACCAGCCTTGCAG
• BKK22580 (ΔreoY::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTAATTCCCTCCTCTAT,  downstream forward: _UP4_TAATATGACCAGCCTTGCAG

References

Research papers

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