yloS

168

thiamine pyrophosphokinase

Locus: BSU_15800

Molecular weight: 23.95 kDa

Isoelectric point: 5.22

Protein length: 214 aa

Gene length: 645 bp

Function: thiamine salvage

Product: thiamine pyrophosphokinase

Essential: no

E.C.: 2.7.6.2

Synonyms: yloS, thiN

Genomic Context

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List of homologs in different organisms, belongs to COG1564 (Galperin et al., 2021)

Gene

Coordinates: 1,654,730  1,655,374

The protein

Catalyzed reaction/ biological activity

• ATP + thiamine --> AMP + H⁺ + thiamine diphosphate (according to UniProt)

Protein family

• thiamine pyrophosphokinase family (single member, according to UniProt)

Structure

• 3LM8 (PDB)
• O34664 (AlphaFold)

Expression and Regulation

Operons

• Genes: prpC-prkC-cpgA-rpeA-yloS

  Description: PubMed

Biological materials

Mutant

• MGNA-B136 (yloS::erm), available at the NBRP B. subtilis, Japan
• BKE15800 (yloS::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCTGATTCCTTTCTCT,  downstream forward: _UP4_TAAGCATGTGCTTTCATTCA
• BKK15800 (yloS::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCTGATTCCTTTCTCT,  downstream forward: _UP4_TAAGCATGTGCTTTCATTCA

References

Reviews

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Original Publications

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