yloM

168

similar to RNA-binding Sun protein, similar to S-adenosyl-L-methionine-dependent 16S rRNA m5C967 methyltransferase

Locus: BSU_15740

Molecular weight: 50.11 kDa

Isoelectric point: 6.95

Protein length: 447 aa

Gene length: 1344 bp

Function: rRNA modification

Product: S-adenosyl-L-methionine-dependent 16S rRNA m5C967 methyltransferase

Essential: no

Synonyms: yloM, sun

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0781 (Galperin et al., 2021)

Gene

Coordinates: 1,647,939  1,649,282

The protein

Catalyzed reaction/ biological activity

• cytidine⁹⁶⁷ in 16S rRNA + S-adenosyl-L-methionine --> 5-methylcytidine⁹⁶⁷ in 16S rRNA + H⁺ + S-adenosyl-L-homocysteine (according to UniProt)

Protein family

• Methyltransferase superfamily (according to UniProt)
• class I-like SAM-binding methyltransferase superfamily (according to UniProt)

Structure

• 1SQF (PDB) (E. coli Fmu protein, complex with SAM) PubMed
• P94464 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: defA-fmt-yloM-yloN

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: yloC-remA-gmk-yloH-coaBC-priA-defA-fmt-yloM-yloN

  Description

  • PubMed

Biological materials

Mutant

• MGNA-B373 (yloM::erm), available at the NBRP B. subtilis, Japan
• BKE15740 (yloM::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGCCTCAAGGGCGATGTCAC,  downstream forward: _UP4_AGCATGAGAAAGAAGGGATA
• BKK15740 (yloM::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGCCTCAAGGGCGATGTCAC,  downstream forward: _UP4_AGCATGAGAAAGAAGGGATA

References

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