yjiC

168

UDP-dependent glycosyltransferase, macrolide glycosyltransferase

Locus: BSU_12220

Molecular weight: 43.83 kDa

Isoelectric point: 4.65

Protein length: 392 aa

Gene length: 1179 bp

Function: synthesis of bacillaene

Product: UDP-dependent macrolide glycosyltransferase

Essential: no

Synonyms: yjiC

Genomic Context

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List of homologs in different organisms, belongs to COG1819 (Galperin et al., 2021)

Gene

Coordinates: 1,292,557  1,293,735

The protein

Protein family

• UDP-glycosyltransferase family (with YdhE and YojK, according to UniProt)

Structure

• 7BOV (PDB) PubMed
• 2IYA (PDB) (from Streptomyces antibioticus, 37% identity) PubMed
• O34539 (AlphaFold)

Expression and Regulation

Operons

• Genes: yjiB-yjiC

  Description: PubMed

Biological materials

Mutant

• MGNA-A345 (yjiC::erm), available at the NBRP B. subtilis, Japan
• BKE12220 (yjiC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAATCTCCAGTCTCCTT,  downstream forward: _UP4_TAAAAACATAAAAACCGAAA
• BKK12220 (yjiC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAATCTCCAGTCTCCTT,  downstream forward: _UP4_TAAAAACATAAAAACCGAAA

References

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