yhfS

168

similar to acetyl-CoA C-acetyltransferase

Locus: BSU_10350

Molecular weight: 38.24 kDa

Isoelectric point: 5.12

Protein length: 364 aa

Gene length: 1095 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yhfS

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0183 (Galperin et al., 2021)

Gene

Coordinates: 1,109,388 → 1,110,482

The protein

Protein family

• thiolase-like superfamily (according to UniProt)

Structure

• 4E1L (PDB) (from Clostridium Difficile, 38% identity)
• 3SS6 (PDB) (the protein of B. anthracis, 38% identity, 66% similarity)
• O07618 (AlphaFold)

Paralogous protein(s)

• FadA, MmgA

Expression and Regulation

Operons

• Genes: panU-yhfT-yhfS-yhfR

  Description: PubMed

  Regulation

  • repressed in the presence of biotin (BirA) PubMed

  Regulatory mechanism

  • BirA: repression, PubMed, in birA regulon

  Additional information

  • weakly expressed PubMed

• Genes: yhfT-yhfS-yhfR

  Description: PubMed

  Additional information

  • weakly expressed PubMed

Biological materials

Mutant

• MGNA-B279 (yhfS::erm), available at the NBRP B. subtilis, Japan
• BKE10350 (ΔyhfS::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATTCATTTATACTGTACACT,  downstream forward: _UP4_TAGGCTTTTTCATAGGACAC
• BKK10350 (ΔyhfS::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATTCATTTATACTGTACACT,  downstream forward: _UP4_TAGGCTTTTTCATAGGACAC

References

Loading