thrB

168

homoserine kinase

Locus: BSU_32240

Molecular weight: 33.17 kDa

Isoelectric point: 4.74

Protein length: 309 aa

Gene length: 930 bp

Function: biosynthesis of threonine

Product: homoserine kinase

Essential: no

E.C.: 2.7.1.39

Synonyms: thrB, thrA

Genomic Context

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List of homologs in different organisms, belongs to COG0083 (Galperin et al., 2021)

Gene

Coordinates: 3,312,844  3,313,773

The protein

Catalyzed reaction/ biological activity

• ATP + L-homoserine --> ADP + H⁺ + O-phospho-L-homoserine (according to UniProt)

Protein family

• GHMP kinase family (together with GalK and IspE) (according to UniProt)

Structure

• 3HUL (PDB) (from Listeria monocytogenes, 41% identity, 61% similarity)
• P04948 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: yutH-hom-thrC-thrB

  Description: PubMed

  Regulation

  • expressed during sporulation PubMed

• Genes: hom-thrC-thrB

  Description: PubMed

  Regulation

  • expressed in the presence of lysine or cysteine (ThrR) PubMed

  Regulatory mechanism

  • CodY: repression, PubMed, in codY regulon
  • TnrA: repression, PubMed, in tnrA regulon
  • ThrR: repression, PubMed, in thrR regulon

Biological materials

Mutant

• BKE32240 (thrB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AGAGAACAGCATGTCGGCTT,  downstream forward: _UP4_TAGATCATGCCAAGCGTTCC
• BKK32240 (thrB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AGAGAACAGCATGTCGGCTT,  downstream forward: _UP4_TAGATCATGCCAAGCGTTCC
• BP1275 (thrB::phleo trp+) available in Jörg Stülke's & Fabian Commichau's lab

References

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