rbfA

168

ribosome-binding factor A

Locus: BSU_16650

Molecular weight: 13.25 kDa

Isoelectric point: 9.34

Protein length: 117 aa

Gene length: 354 bp

Function: translation

Product: ribosome-binding factor A

Essential: no

Synonyms: rbfA, ymxE, ylxO

Genomic Context

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List of homologs in different organisms, belongs to COG0858 (Galperin et al., 2021)

Gene

Coordinates: 1,736,451 → 1,736,804

The protein

Protein family

• rbfA family (single member, according to UniProt)

Structure

• 1JOS (PDB) (from Haemophilus influenzae, 45% identity)
• P32731 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ylxS-nusA-rnpM-rplGA-infB-ylxP-rbfA

  Description: PubMed

  Regulation

  • autoregulation NusA PubMed
  • induced by glucose PubMed
  • expression is reduced in motile cells as compared to non-motile cells PubMed

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response
  • NusA: attenuation, NusA stimulates termination PubMed, in nusA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigB: sigma factor, PubMed, in sigB regulon

Biological materials

Mutant

• BKE16650 (ΔrbfA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACTCAATTCAATCACCT,  downstream forward: _UP4_TAAAAAAAGAGAGGATAGGC
• BKK16650 (ΔrbfA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACTCAATTCAATCACCT,  downstream forward: _UP4_TAAAAAAAGAGAGGATAGGC

References

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