rapG

168

response regulator aspartate phosphatase, controls DegU activity

Locus: BSU_40300

Molecular weight: 42.68 kDa

Isoelectric point: 4.81

Protein length: 365 aa

Gene length: 1098 bp

Function: control of DegU activity

Product: response regulator aspartate phosphatase

Essential: no

Synonyms: rapG, yycM

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 4,140,260 → 4,141,357

The protein

Catalyzed reaction/ biological activity

• control of DegU activity PubMed

Protein family

• RAP family (according to UniProt)

Domains

• six tetratrichopeptide repeats (according to UniProt)

Structure

• 8RTE (PDB) (phi 105 RAP phosphatase, 39.3% identity) PubMed
• 4I9E (PDB) (RapF, 26% identity) PubMed
• O32294 (AlphaFold)

Effectors of protein activity

• binding of PhrG to RapG prevents it from binding DegU PubMed

Expression and Regulation

Operons

• Genes: rapG-phrG

  Description: PubMed

  Regulation

  • repressed by glucose (4.8-fold) (CcpA) PubMed

  Regulatory mechanism

  • SinR: repression, PubMed, in sinR regulon
  • RghR: repression, PubMed, in rghR regulon
  • CcpA: repression, PubMed, in ccpA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigH: sigma factor, PubMed, in sigH regulon

Biological materials

Mutant

• MGNA-B821 (yycM::erm), available at the NBRP B. subtilis, Japan
• BKE40300 (ΔrapG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTGAAACTCCTTTCTCT,  downstream forward: _UP4_ATTAAACGAACGGAGGTTAT
• BKK40300 (ΔrapG::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTGAAACTCCTTTCTCT,  downstream forward: _UP4_ATTAAACGAACGGAGGTTAT

References

Reviews

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Original Publications

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