mhqD

168

may be involved in protection against methyl-hydroquinone

Locus: BSU_19560

Molecular weight: 22.29 kDa

Isoelectric point: 5.62

Protein length: 200 aa

Gene length: 603 bp

Function: may be involved in protection against methyl-hydroquinone

Product: unknown

Essential: no

Synonyms: mhqD, yodD

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0400 (Galperin et al., 2021)

Gene

Coordinates: 2,128,464  2,129,066

The protein

Protein family

• AB hydrolase superfamily (according to UniProt)

Structure

• 2H1I (PDB) (from B. cereus, 59% identity)
• O34842 (AlphaFold)

Expression and Regulation

Operons

• Genes: mhqE-mhqD

  Description: PubMed

  Regulation

  • induced by 2-methylhydroquinone (MhqR) PubMed

  Regulatory mechanism

  • MhqR: repression, PubMed, in mhqR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-B435 (yodD::erm), available at the NBRP B. subtilis, Japan
• BKE19560 (mhqD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATGCTTCATTATAAATTCCC,  downstream forward: _UP4_TAAAAAAACCTTAGTCCGGA
• BKK19560 (mhqD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATGCTTCATTATAAATTCCC,  downstream forward: _UP4_TAAAAAAACCTTAGTCCGGA

References

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