lytG

168

peptidoglycan N-acetylglucosaminidase

Locus: BSU_31120

Molecular weight: 31.75 kDa

Isoelectric point: 8.28

Protein length: 282 aa

Gene length: 849 bp

Function: cell wall turnover

Product: peptidoglycan N-acetylglucosaminidase

Essential: no

Synonyms: lytG, yubE

Genomic Context

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List of homologs in different organisms, belongs to COG1705 (Galperin et al., 2021)

Gene

Coordinates: 3,190,834 → 3,191,682

The protein

Catalyzed reaction/ biological activity

• hydrolysis of the glycosidic bond between N-acetyl-β-d-glucosamine residues and adjacent monosaccharides PubMed

Protein family

• glycosyl hydrolase 73 family (with LytD, according to UniProt)

Domains

• GW domain (aa 203-281) (according to UniProt)

Structure

• O32083 (AlphaFold)

Localization

• secreted (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: lytG-yubF

  Description: PubMed

Biological materials

Mutant

• MGNA-A027 (yubE::erm), available at the NBRP B. subtilis, Japan
• BKE31120 (ΔlytG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAAAACCTTCCTTCGAA,  downstream forward: _UP4_TAAGCCCTTTTTACTGGTAT
• BKK31120 (ΔlytG::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGAAAACCTTCCTTCGAA,  downstream forward: _UP4_TAAGCCCTTTTTACTGGTAT

References

Reviews

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Original Publications

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