lytC

168

major autolysin, N-acetylmuramoyl-L-alanine amidase, required for lateral cell wall growth and for flagellar function

Locus: BSU_35620

Molecular weight: 52.46 kDa

Isoelectric point: 10.11

Protein length: 496 aa

Gene length: 1491 bp

Function: major autolysin, cell separation, wall turnover

Product: N-acetylmuramoyl-L-alanine amidase

Essential: no

E.C.: 3.5.1.28

Synonyms: lytC, cwlB

Genomic Context

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List of homologs in different organisms, belongs to COG2247 (Galperin et al., 2021)

Gene

Coordinates: 3,659,119  3,660,609

Phenotypes of a mutant

• impaired in motility, the phenotype is suppressed by mutations in ''lonA or smiA'' PubMed

The protein

Catalyzed reaction/ biological activity

• Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides (according to UniProt)

Protein family

• N-acetylmuramoyl-L-alanine amidase 3 family (according to UniProt)

Domains

• contains an amidase_3 domain (like CwlC, CwlD, YqiI, YrvJ)
• MurNAc-LAA domain (aa 322-490) (according to UniProt)

Structure

• 5J72 (PDB) (from Clostridium difficile, 32% identity) PubMed
• Q02114 (AlphaFold)

Localization

• secreted (according to Swiss-Prot)
• for an analysis of motion of LytC, see PubMed

Expression and Regulation

Operons

• Genes: lytA-lytB-lytC

  Description: PubMed

  Expression

  • expression is heterogeneous PubMed

  Regulatory mechanism

  • SinR: repression, in sinR regulon
  • YvrHb: repression, PubMed, in yvrHb regulon
  • SlrR: repression, (in complex with SinR) PubMed, in slrR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigD: sigma factor, PubMed, in sigD regulon

Biological materials

Mutant

• 1A789 ( lytC::kan), PubMed, available the Bacillus Genetic Stock Center
• BKE35620 (lytC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATTGATTTCCTCCTTAAA,  downstream forward: _UP4_TAATCGAAAGAGACAAATCT
• BKK35620 (lytC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATTGATTTCCTCCTTAAA,  downstream forward: _UP4_TAATCGAAAGAGACAAATCT

References

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