hisZ

168

ATP phosphoribosyltransferase regulatory subunit

Locus: BSU_34930

Molecular weight: 43.34 kDa

Isoelectric point: 5.15

Protein length: 391 aa

Gene length: 1176 bp

Function: biosynthesis of histidine

Product: regulatory subunit for ATP phosphoribosyltransferase (HisG)

Essential: no

Synonyms: hisZ

Genomic Context

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List of homologs in different organisms, belongs to COG3705 (Galperin et al., 2021)

Gene

Coordinates: 3,588,185 → 3,589,360

Phenotypes of a mutant

• auxotrophic for histidine PubMed

The protein

Protein family

• Class-II aminoacyl-tRNA synthetase family (according to UniProt)

Structure

• 3OD1 (PDB) (from B. halodurans, 57% identity)
• O34459 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: hisZ-hisG-hisD-hisB-hisH-hisA-hisF-hisI

  Description: PubMed

  Regulation

  • expression during spore germination is strongly reduced under conditions of osmotic stress PubMed
  • expression depends on functional RnpM PubMed

  Regulatory mechanism

  • RnpM: unknown, PubMed, in rnpM regulon

Biological materials

Mutant

• BKE34930 (ΔhisZ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAACCCGAGCACCTCCG,  downstream forward: _UP4_CAAAATGGGTAAGTTACTCA
• BKK34930 (ΔhisZ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAACCCGAGCACCTCCG,  downstream forward: _UP4_CAAAATGGGTAAGTTACTCA

References

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