hisI

168

phosphoribosyl-AMP cyclohydrolase / phosphoribosyl-ATP pyrophosphohydrolase

Locus: BSU_34860

Molecular weight: 23.75 kDa

Isoelectric point: 4.63

Protein length: 209 aa

Gene length: 630 bp

Function: biosynthesis of histidine

Product: phosphoribosyl-AMP cyclohydrolase /phosphoribosyl-ATP pyrophosphohydrolase

Essential: no

E.C.: 3.5.4.19

Synonyms: hisI, hisIE

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0140 (Galperin et al., 2021)

Gene

Coordinates: 3,582,936  3,583,565

The protein

Catalyzed reaction/ biological activity

• 1-(5-phospho-β-D-ribosyl)-ATP + H₂O --> 1-(5-phospho-β-D-ribosyl)-5'-AMP + diphosphate + H⁺ (according to UniProt)
• 1-(5-phospho-β-D-ribosyl)-5'-AMP + H₂O --> 1-(5-phospho-β-D-ribosyl)-5-[(5-phospho-β-D-ribosylamino)methylideneamino]imidazole-4-carboxamide (according to UniProt)

Domains

• N-terminal domain (aa 1 ... 116): phosphoribosyl-AMP cyclohydrolase domain (according to UniProt) PubMed
• C-terminal domain (aa 117 ... 209): phosphoribosyl-ATP pyrophosphohydrolase domain (according to UniProt)

Structure

• 1ZPS (PDB) (the N-terminal domain, from Methanobacterium thermoautotrophicum, 49% identity)
• 1YVW (PDB) (the C-terminal domain, from B. cereus, 49% identity)
• O34912 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: hisZ-hisG-hisD-hisB-hisH-hisA-hisF-hisI

  Description: PubMed

  Regulation

  • expression during spore germination is strongly reduced under conditions of osmotic stress PubMed
  • expression depends on functional RnpM PubMed

  Regulatory mechanism

  • RnpM: unknown, PubMed, in rnpM regulon

Biological materials

Mutant

• BKE34860 (hisI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAAACGCAGTTCATCTGCCT,  downstream forward: _UP4_TAAAGAGCCGGATGATACGG
• BKK34860 (hisI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AAAACGCAGTTCATCTGCCT,  downstream forward: _UP4_TAAAGAGCCGGATGATACGG

References

Loading