hisH

168

imidazole glycerol phosphate synthase (glutaminase subunit)

Locus: BSU_34890

Molecular weight: 23.16 kDa

Isoelectric point: 5.72

Protein length: 212 aa

Gene length: 639 bp

Function: biosynthesis of histidine

Product: imidazole glycerol phosphate synthase (glutaminase subunit)

Essential: no

Synonyms: hisH

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0118 (Galperin et al., 2021)

Gene

Coordinates: 3,585,051 → 3,585,689

The protein

Catalyzed reaction/ biological activity

• 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamide + L-glutamine --> 5-amino-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamide + D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H⁺ + L-glutamate (according to UniProt)
• H₂O + L-glutamine --> L-glutamate + NH₄⁺ (according to UniProt)

Domains

• Glutamine amidotransferase type-1 domain (aa 1-211) (according to UniProt)

Structure

• 1KA9 (PDB) (the HisF-HisH complex from Thermus thermophilus, 39% identity) PubMed
• O34565 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: hisZ-hisG-hisD-hisB-hisH-hisA-hisF-hisI

  Description: PubMed

  Regulation

  • expression during spore germination is strongly reduced under conditions of osmotic stress PubMed
  • expression depends on functional RnpM PubMed

  Regulatory mechanism

  • RnpM: unknown, PubMed, in rnpM regulon

Biological materials

Mutant

• BKE34890 (ΔhisH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TCCGTAATCTATCACGCCGA,  downstream forward: _UP4_GCAGCAGAACAGAAGGTGAA
• BKK34890 (ΔhisH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TCCGTAATCTATCACGCCGA,  downstream forward: _UP4_GCAGCAGAACAGAAGGTGAA

References

Reviews

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Original Publications

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